Density gradient outperforms swim-up when examining different sperm nuclear DNA integrity and membrane characteristics
نویسندگان
چکیده
منابع مشابه
Sperm DNA fragmentation and sex chromosome aneuploidy after swim-up versus density gradient centrifugation
Objective The aim of this study was to compare the efficacy of swim-up and density gradient centrifugation (DGC) for reducing the amount of sperm with fragmented DNA, sex chromosome aneuploidy, and abnormal chromatin structure. Methods Semen samples were obtained from 18 healthy male partners who attended infertility clinics for infertility investigations and were processed with swim-up and D...
متن کاملcan zeta sperm selection method, recover sperm with higher dna integrity compare to density gradient centrifugation?
background: sperm selection for icsi based on morphology and motility might not be relevant to chromatin integrity. thus sperm selection based on sperm characteristics has been suggested. objective: the aim of this study was to compare the efficiency of zeta method with routine density gradient centrifugation method (dgc) for the selection of sperm with higher dna integrity. materials and metho...
متن کاملComparison of the DNA Fragmentation and the Sperm Parameters after Processing by the Density Gradient and the Swim up Methods.
INTRODUCTION The swim up and the density-gradient centrifugation are the two main techniques which are used to separate the viable motile sperm fraction in the assisted reproductive technology. However, there are several published studies about these methods, but there is no sufficient evidence for recommending the superiority of one of them. This study was designed to study the efficiency of t...
متن کاملSemen preparation methods and sperm telomere length: density gradient centrifugation versus the swim up procedure
Previous studies have shown that both density gradient centrifugation (DGC) and swim up (SU) procedures can select spermatozoa with longer telomeres for assisted reproduction techniques (ART). However, it is unknown which approach is more effective. The aim of the present study was to compare the effects of these two methods on sperm telomere length (STL). A total of 150 normozoospermic subject...
متن کاملEffect of swim-up sperm washing and subsequent capacitation on acrosome status and functional membrane integrity of normal sperm.
OBJECTIVE Sperm preparation techniques select sperm population with improved sperm motion characteristics. We sought to determine whether the swim-up technique selects spermatozoa with the ability to undergo hypoosmotic swelling, and how swim-up and subsequent capacitation affect the acrosome reaction rate. METHODS Semen specimens from 15 normal donors were divided into unprocessed, swim-up, ...
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ژورنال
عنوان ژورنال: Fertility and Sterility
سال: 2018
ISSN: 0015-0282
DOI: 10.1016/j.fertnstert.2018.07.962